X-Gal5-溴-4-氯-3-吲哚基 b-D-吡喃半乳糖苷

Chromogenic substrate for β-galactosidase generating a blue compound after galactosidase hydrolysis. X-Gal is the substrate of choice in cloning and genetic engineering for selection of recombinant bacterial colonies with the lac+ genotype. It has application in immunohistochemistry.

Technical Data

CAS号: 7240-90-6
别名: 5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside (X-Gal); X-b-D-Galactoside; BCIG
产品编码: EB06680
MDL号: MFCD00005666
分子式: C14H15BrClNO6
分子量: 408.63 g/mol


  • Arakawa H et al (1991). Chemiluminescent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay. Anal Biochem 199(2):238-42., Weiss DJ et al (1997). In situ histochemical detection of beta-galactosidase activity in lung: assessment of X-Gal reagent in distinguishing lacZ gene expression and endogenous beta-galactosidase activity. Human Gene Therapy 8(13):1545-1554.
  • Engler KH et al (1999). A novel colorimetric yeast bioassay for detecting trichothecene mycotoxins. J Microbiol Methods 35(3):207-18.
  • Joung JK et al (2000). A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions. Proc Natl Acad Sci U S A 97(13):7382-7.
  • Sambrook J and Russell DW (2001). Molecular Cloning: A Laboratory Manual, 3rd Edition, Cold Spring Harbor Laboratory Press.
  • Seymour PA and Sander M (2007). Immunohistochemical detection of beta-galactosidase or green fluorescent protein on tissue sections. Methods Mol Biol 411:13-23.



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